Variant isoforms of CD44 are required in early thymocyte development.
作者： Christoph Schwärzler , Snezhana Oliferenko , Ursula Günthert
DOI: 10.1002/1521-4141(2001010)31:10<2997::AID-IMMU2997>3.0.CO;2-J
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摘要: The earliest T cells homing to the thymus (CD3-CD4loCD8-) express CD117 (c-kit), CD43 (leukosialin), and integrins CD11a (alphaL), CD11b (alphaM), CD29 (beta1), CD49f (alpha6), CD44. Using reagents specific for CD44 variant isoforms (CD44v), we demonstrated that CD44v were expressed on virtually all early thymocytes,whereas carrying only standard molecule (CD44s, not containing any domains), which is ubiquitously found mature lymphocytes later, are very sparse. expression of was closely correlated with restricted CD3-CD4loCD8- stage. detected lymphocyte progenitor populations in fetal blood, liver, spleen, as well adult bone marrow. Functional studies expressing from liver marrow could efficiently populate thymic stroma develop into cells. In organ cultures anti-CD44v antibodies specifically blocked thymocyte development. We also present evidence required initial interaction hematopoietic stroma. Our data imply a useful marker progenitors, but play functional role initiation
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