Osteogenic Potential of Human Oral-Periosteal Cells (PCs) Isolated From Different Oral Origin: An In Vitro Study.

摘要: The periosteum is a specialized connective tissue containing multipotent stem cells capable of bone formation. In this study, we aimed at demonstrating that human oral periosteal derived from three different sites (upper vestibule, lower and hard palate) represent an innovative cell source for maxillo-facial engineering applications in terms accessibility self-commitment towards osteogenic lineage. Periosteal (PCs) were isolated patients with ages (20-30 yy, 40-50 50-60 yy); then analyzed the vitro proliferation capacity clones culturing them without any supplement to support their differentiation. We found PCs, independently origin age patients, are mesenchymal characteristics (clonogenical proliferative activity) that, even absence induction, they undertake osteoblast lineage after 45 days culture. These results suggest could replace marrow tissue-engineering applications.