The Hippo Pathway Effector YAP1 Regulates Intestinal Epithelial Cell Differentiation.
作者： Sepideh Fallah , Jean-François Beaulieu
DOI: 10.3390/CELLS9081895
关键词:
摘要: The human intestine is covered by epithelium, which continuously replaced new cells provided stem located at the bottom of glands. maintenance intestinal supported a niche composed several signaling proteins including Hippo pathway effectors YAP1/TAZ. role YAP1/TAZ in cell proliferation and regeneration well documented but their involvement on differentiation epithelial unclear. In present study, was investigated using HT29 line, only multipotent line available, with combination knockdown approaches. expression markers tested qPCR, Western blot, indirect immunofluorescence electron microscopy analyses. results show that TAZ not expressed while abolition YAP1 led to sharp increase goblet absorptive reduction some markers. Further studies double experiments revealed most these effects resulting from are mediated CDX2, key transcription factor. conclusion, our indicate negatively regulate through inhibition CDX2 expression.
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