An outbreak of African Swine Fever in Nigeria: virus isolation and molecular characterization of the VP72 gene of a first isolate from West Africa.

摘要: The isolation of 98/ASF/NG, a strain African Swine Fever Virus (ASFV) associated with 1998 epizootic in Nigeria, is reported. This first isolate the virus from West Africa was identified through successful polymerase chain reaction (PCR) amplification and sequencing 280 base pair (bp) fragment Major Capsid Protein (VP72) gene. Further sequence analysis 1.9 kilobase (kbp) encompassing complete VP72 gene showed that has 92.2%, 92.4%, 97.2% homology previously sequenced Ugandan, Dominican Republican Spanish isolates respectively. Of 50 nucleotide changes observed this highly conserved gene, 45 were found to result 40 amino acid clustered around central region (position 426 516) VP 72 protein while at remaining 5 positions silent. These also led loss two out seven potential N-glycosylation sites which are among all isolates. possible epizootiological implications such mutations DNA discussed relation outbreak.