Molecular Biology of Insect Iridescent Virus Type 6

摘要: The genome of the insect iridescent virus type 6-Chilo (CIV)— contains a double-stranded linear DNA molecule 209 kbp which is circularly permuted and terminally redundant. A defined complete gene library viral was established represents 100% CIV sequences. physical maps were constructed for restriction enzymes ApaI, Asp718, BamHI, EcoRI, NcoI, PvuII, SalI, SphI, SmaI. Although are circular due to permutation molecule. repetitive sequences located in EcoRI fragments H PvuII fragment L (5064 bp) at coordinates 0.535 0.548 0.920 0.944, respectively. element (91 nucleotide position 1981 2072 had been found be complementary (>90%) nine regions L. stem-loop structure has identified by heteroduplex mapping 0.571 0.582 (HindIII/EcoRI subfragment (2555 H). sequence many perfect direct repeats sizes up 145 bp. In addition these repetitions cluster four imperfect elements (R1 R4) with complex structural arrangement detected. R1, R2 R3 exist duplicate (two boxes (B)) between positions 271 3466). R4 12 (between bases 1301 4417). Five open reading frames (ORFs 118 333 amino acid (AA) residues) analysis largest ORF revealed that deduced putative product two repetitions. Sequences 43 residues 5 (160 202 AA) homologous within majority ORFs. consensus sequence-MANL (X)6 IGSSST(X)6 L(X)1 LGS(X)1 LQISG(X)2 VN- all five origins replication C (13.5 kbp, 0.909 0.974 map units (m.u.)), (9.8 m.u.), M (7.3 0.310 0.345 0 (6.6 0.196 0.228 Q (5.9 0.603 0.631 Y (2.0 0.381 0.391 m.u.).