Comparative transcriptome analysis reveals defense-related genes and pathways against downy mildew in Vitis amurensis grapevine.

摘要: Abstract Downy mildew (DM), caused by oomycete Plasmopara viticola (Pv), can lead to severe damage Vitis vinifera grapevines. amurensis has generally been regarded as a DM resistant species. However, when V. amurensis ‘Shuanghong’ were inoculated with Pv strains ‘ZJ-1-1’ and ‘JL-7-2’, the former led obvious symptoms (compatible), while latter did not develop any but exhibited necrosis (incompatible). In order underlie molecular mechanism in resistance, mRNA-seq based expression profiling of was compared at 12, 24, 48 72 h post inoculation (hpi) these two strains. Specific genes their corresponding pathways responsible for incompatible interaction extracted comparing compatible interaction. interaction, 37 resistance (R) more expressed early stage infection (12 hpi). Similarly, involved defense signaling, including MAPK. ROS/NO, SA, JA, ET ABA pathways, associated defense-related metabolites synthesis, such pathogenesis-related phenylpropanoids/stilbenoids/flavonoids biosynthesizing genes, also activated mainly during stages infection. On other hand, Ca2+ signaling primary metabolism, photosynthesis fatty acid repressed after ‘JL-7-2’ challenge. Further quantification some key factors, phytohormones, phytoalexins ROS, showed much accumulation indicating important roles defense. addition, total 43 52 RxLR effectors detected processes, respectively.