Rejection of mouse sarcoma cells after transfection of MHC class II genes.

摘要: Th cells are stimulated by peptide Ag presented in the context of MHC class II molecules. We have reasoned that immune responses against tumors may be more efficient if tumor were positive, and thereby able to directly function as APC stimulate tumor-specific cell proliferation. tested this hypothesis using DNA-mediated gene transfer generate syngeneic Ag-expressing mouse Sal sarcoma (Sal/Ak transfectants). Autologous A/J mice challenged i.p. or s.c. with Sal/Ak transfectants do not develop tumors, whereas negative parental a high incidence. Furthermore, immunization autologous host completely protects subsequent challenge wild-type cells. II-expressing cells, therefore, an improved response, immunity is cross-reactive tumor. Inasmuch transfected product functioning target molecule for rejection, immunogenicity probably due stimulation population. The increased is, result direct presentation tumor-associated molecules lymphocytes. These studies demonstrate induction expression potential strategy immunotherapy, suggest enhanced generation.