Diagnostic and prognostic significance of image cytometric DNA ploidy measurement in cytological samples of cervical squamous intraepithelial lesions.

摘要: D. Demirel, N. Akyurek and I. Ramzy Diagnostic prognostic significance of image cytometric DNA ploidy measurement in cytological samples cervical squamous intraepithelial lesions Objective:  To study the pattern uterine lesions (SILs) its diagnostic significance. Methods:  The included 31 cases SIL: 11 low-grade (LSIL) 20 high-grade (HSIL). Feulgen–pararosaniline staining was performed on previously Papanicolaou-stained smears a performed. An internal reference used to calibrate samples. Results:  All SIL, either LSIL or HSIL, were non-diploid. Of LSIL, four tetraploid seven aneuploid, whereas, 16 aneuploid. Stemline aneuploidy not significant discriminator between HSIL (P = 0.32). Based single-cell analysis, had significantly higher content than (P < 0.01). When mean 30% more for 6c-exceeding event (6cEE) value, sensitivity specificity indicate 83% 64%, respectively, with positive predictive value (PPV) 81% negative (NPV) 65%. neoplasia grade 2 worse (CIN2+) biopsy. In addition, which showed recurrence by analysis those an indolent clinical behaviour: P = 0.04 P = 0.03 respectively. Conclusions:  Image is useful technique purposes SIL when appropriate ‘c’ values are analysis. We propose that >6c as cut-off level predicting CIN2+ cytometry smears.