The Addiction-Related Gene Ankk1 is Oppositely Regulated by D1R- and D2R-Like Dopamine Receptors

摘要: The ankyrin repeat and kinase domain containing 1 (ANKK1) TaqIA polymorphism has been extensively studied as a marker of the gene for dopamine receptor D2 (DRD2) in addictions other dopamine-associated traits. In vitro mRNA protein studies have shown potential connection between ANKK1 dopaminergic system functioning. Here, we investigated whether Ankk1 expression brain is regulated by treatment with agonists. We used quantitative RT-PCR total Western blots specific areas to study murine after treatments. found that was upregulated activation D1R-like receptors SKF38393 (2.660 ± 1.035-fold; t: 4.066, df: 11, P = 0.002) apomorphine (2.043 ± 0.595-fold; 3.782, 8, P = 0.005). D2R-like agonist quinelorane no effect upon (1.004 ± 0.580-fold; 0.015, 10, P = 0.9885). contrast, mice agonists 7-OH-DPAT aripiprazole caused significant downregulation (0.606 ± 0.057-fold; 2.786, P = 0.02 0.588 ± 0.130-fold; 2.394, P = 0.036, respectively). With respect proteins profile, effects were (t: 0.54, 2, P = 0.643) Quinelorane 0.286, P = 0.782) (±) increment striatum 2.718, 7; P = 0.03) when compared prefrontal cortex. D2-R-like leads opposite transcriptional regulation pathways.