Phenotypic and Molecular Assessment of Antimicrobial Resistance in Lactobacillus paracasei Strains of Food Origin

摘要: Antimicrobial resistance data in food-associated lactic acid bacteria (LAB) such as lactobacilli are mostly based on nonstandardized methodologies and/or have been obtained for only a limited number of strains. This susceptibility study included diverse collection 115 isolates mainly food origin originally identified Lactobacillus paracasei or casei. Upon reidentification and removal potential replicate using repetitive DNA element PCR fingerprinting, 65 genotypically unique L. strains the casei type strain were selected broth microdilution Etest assays LAB test medium. In both methodologies, appeared uniformly susceptible to ampicillin clindamycin but exhibited natural streptomycin gentamicin. Three from cheese displayed acquired tetracycline (MIC ≥ 32 μg/ml) erythromycin >16 μ g/ml), which was linked presence tet(M) tet(W) g...