Identification of Genes Differentially Expressed in the Ganglia of GrowingHaliotis asinina

摘要: There is currently a paucity of specific genetic data regarding growth and reproduction-related processes in abalone, marine vetigastropods commercial value. However, strong inferences about these can be drawn from other molluscs. For example, ganglia the gastropods Aplysia Lymnaea are known to produce neuropeptides related growth, feeding behavior, reproduction. Here, we use suppression subtractive hybridization identify expressed genes that may linked control tropical abalone Haliotis asinina. Two pools clones were obtained messenger RNA derived H. asinina cerebral pleuropedal ganglia, corresponding differentially (1) well-nourished animals relative food-deprived ones (2) reproductively active ones. From subtractions, respectively, 204 214 unigenes identified 222 231 sequenced clones, with 18 common both subtractions. A subset putative was confirmed by quantitative polymerase chain reaction, validating this approach. The transcripts growing fall into wide range functional categories, including biological regulation, cell proliferation, metabolic process, include encoding Phe-Met-Arg-Phe-NH2 (or, FMRF-amide), myomodulin, CDC123, RAB37, dermatopontin.