Influence of the Temperature and the Genotype of the HSP90AA1 Gene over Sperm Chromatin Stability in Manchega Rams

摘要: The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated sperm DNA fragmentation (DFI) by SCSA ejaculates incubated at 37°C during 0, 24 and 48 hours after its collection, as a way to mimic temperature circumstances which spermatozoa will be subject in ewe uterus. effects temperature-humidity index (THI) from day 60 prior collection date semen DFI were examined. To better understand causes determining sensitivity heat, this was conducted males with alternative genotypes for SNP G/C−660 HSP90AA1 promoter, encode Hsp90α protein. protein predominates brain testis, role spermatogenesis has been described several species. Ridge regression analyses showed that days 29 35 7 14 before (bsc) most critical regarding over values. Mixed model revealed increases threshold 30°C maximum 22 THI bsc only animals carrying GG−660 genotype. period 29–35 coincide meiosis I process mice. 7–14 may correspond later stages II early epididymal maturation replacement histones protamines occurs. Because genotype associated lower levels expression, suboptimal amounts mRNA under conditions make more susceptible fragmented. Thus, selecting against could decrease thermal susceptibility season, putative subsequent fertility gains.