Expression of HSP105 and HSP60 during germ cell apoptosis in the heat-treated testes of adult cynomolgus monkeys (macaca fascicularis)

摘要: To confirm that transient increase in temperature of the testis (43C for 30 minutes once daily 2 consecutive days) could induce apoptosis germ cells non-human primates and to investigate possible roles Hsp105 Hsp60 regulation cell loss, we conducted study on eight cynomolgus monkeys. The sperm concentration day 28 after heat shock decreased 8.4% pretreatment levels recovered baseline 144. Using TUNEL assay, increased numbers apoptotic spermatocytes round spermatids were detected days 3, 8, post treatment. mRNA protein analyzed using situ hybridization, RT-PCR, immunohistochemical Western blot methods. was confined nuclei before treatment, dramatically with loss 30, returning 84 expression high only Sertoli spermatogonia. These results suggested exposure resulted selective, but reversible damage seminiferous epithelium via apoptosis. Temporal changes pattern relation death suggests they may be involved key processes