2'-anthraquinone-conjugated oligonucleotide as an electrochemical probe for DNA mismatch.
作者： Satoshi Kumamoto , Mariko Watanabe , Naoko Kawakami , Mitsunobu Nakamura , Kazushige Yamana
DOI: 10.1021/BC070097F
关键词:
摘要: We previously prepared the oligonucleotides (ODNs) conjugated to an anthraquinone (AQ) group via one carbon linker at 2′-sugar position. When these modified ODNs bind cDNA sequences, AQ moiety can be intercalated into predetermined base-pair pocket of a duplex DNA. In this paper, 2′-AQ-modified are shown excellent electrochemical probe clarify effect mismatch base on charge transfer (CT) though Two types DNA-modified electrodes were constructed by assembly disulfide-terminated 2′-AQ-ODN duplexes onto gold electrodes. One type (system I) contains fully matched pairs or single-base in DNA between redox center and electrode. The other II) consists but outside center. analyzed cyclic voltammetry estimate CT rate through system I, was found ∼50 s−1 for AQ-ODN duplexes, while ...
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