Changes in lymphocyte subsets, mitogen responsiveness, and interleukin-2 production after cardiac operations

摘要: We studied 104 patients after cardiac operations to search for changes in peripheral blood lymphocyte subsets, mitogen responsiveness, and interleukin-2 production. Mononuclear cells obtained from 10 with cholecystectomy were examined as control samples. Cultures phytohemagglutinin production incubated microtiter plates 24 hours labeled tritium thymidine used target cells. Lymphocyte subsets measured by using monoclonal antibodies. The results preoperative samples compared those the first, third, seventh days operation. In having operations, responsiveness was low on both first operation markedly depressed (greater than 90% depressed) postoperative day. However, cholecystectomy, showed no significant changes. Improvement occurred immediately without transfusion random donors reached normal levels day 3. remained 3 all significantly diminished even 7 such New York Heart Association classes III IV. conclude that heart which cardiopulmonary bypass is are associated depression of cellular immunity, including Furthermore, it appears general condition contribute suppression measures cell-mediated immunity.