An interferon-inducible molecule on brain endothelium which controls lymphocyte adhesion mediated by integrins.

摘要: We undertook a search for cytokine-inducible molecules present on brain endothelium and which are involved in the control of lymphocyte adhesion. screened 39 monoclonal antibodies (mAb) against rat vitro, identified five recognizing molecules. None blocked adhesion, but one antibody (4A2), produced 400% enhancement binding. The 4A2 antigen is induced by interferon-gamma (INF-gamma) not tumour necrosis factor-alpha (TNF-alpha), at 6-48 hr. It preferentially expressed near inter-endothelial cell junctions, it also all lymphocytes weakly aortic vitro. In vivo, detectable cells normal central nervous system (CNS), however appears CNS during T-cell mediated immune reactions. Triggering via this molecule enhances integrin-mediated adhesion to endothelium, primarily LFA-1. Unlike ICAM-1, both Lewis PVG strains. Although, has some functional properties human CD31, CD31. cellular localization molecule, its actions induction IFN-gamma, indicate that recognizes migration into brain.