Cell growth on immobilized cell growth factor. 9. Covalent immobilization of insulin, transferrin, and collagen to enhance growth of bovine endothelial cells

作者: Shu Qin Liu , Yoshihiro Ito , Yukio Imanishi

DOI: 10.1002/JBM.820270710

关键词:

摘要: Bovine endothelial cells were cultivated on the surface of polyurethane membranes or tubes immobilized with insulin, transferrin, and/or collagen, and growth was measured. For purpose immobilization, amino groups introduced by glow-discharge treatment in presence ammonia gas. The proteins coupled to surfaces using dimethyl suberimidate (DMS) water-soluble carbodiimide (WSC). Growth bovine higher insulin transferrin DMS than that WSC. cell free respectively. Though collagen did not affect growth, coimmobilization brought about greater acceleration growth. In addition, indispensible maintain material for a long time. grown tubes, which are coimmobilized, kept normal cobblestone-like appearance maintained ability secrete prostacyclin 9 months.

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