Isolation and characterization of a vascular endothelial cell mitogen produced by pituitary-derived folliculo stellate cells.

摘要: A growth factor with specificity for vascular endothelial cells has been identified in conditioned medium of pituitary-derived folliculo stellate cells. This factor, named stellate-derived (FSdGF), was purified to homogeneity by a combination heparin-Sepharose affinity chromatography, Bio-Gel P-60 exclusion Mono S ion-exchange and hydrophobic chromatography on C4 reverse-phase HPLC column. FSdGF characterized as homodimer composed two subunits molecular mass 23 kDa. potent mitogen activity detectable at 25 pg/ml saturation 500 pg/ml. It did not stimulate the proliferation other cell types such bovine smooth muscle cells, corneal adrenal cortex granulosa BALB/MK or BHK-21 Microsequencing revealed an N-terminal sequence having no significant homology any known protein. The release pituitary its target raise possibility that may play role angiogenesis.