A human protein selected for interference with Ras function interacts directly with Ras and competes with Raf1.

摘要: The overexpression of some human proteins can cause interference with the Ras signal transduction pathway in yeast Saccharomyces cerevisiae. functional block is located at level effector itself, since these do not suppress activating mutations further downstream same pathway. We now demonstrate, vivo and vitro experiments, that protein encoded by one cDNA (clone 99) interact directly Ras2p H-Ras protein, we have named this gene rin1 (Ras interaction/interference). interaction between Rin1 enhanced when bound to GTP. able either an mutant or a dominant negative H-Ras. Thus, displays profile as seen for Raf1 adenylyl cyclase, two known effectors Ras. Moreover, competes binding vitro. Unlike Raf1, however, resides primarily plasma membrane, where localized. These data are consistent functioning mammalian cells regulator