Bioprofiling of Salvia miltiorrhiza via planar chromatography linked to (bio)assays, high resolution mass spectrometry and nuclear magnetic resonance spectroscopy.

摘要: Abstract An affordable bioanalytical workflow supports the collection of data on active ingredients, required for understanding health-related food, superfood and traditional medicines. Targeted effect-directed responses single compounds in a complex sample highlight this powerful hyphenation planar chromatography with (bio)assays. Among many reports about biological properties Salvia miltiorrhiza Bunge root (Danshen) their analytical methods, highly efficient direct bioautography (DB) has not been considered so far. There was just one TLC-acetylcholinesterase (AChE) method poor zone resolution apart from our two HPTLC-DB studies, however, all methods were focused nonpolar extracts Danshen (tanshinones) only. The current study HPTLC-UV/Vis/FLD-(bio)assay-HRMS, followed by streamlined scale-up to preparative layer (PLC)-1H-NMR, aimed at an even more streamlined, yet comprehensive workflow. It comprised screening both, its polar (containing phenolics) tanshinones) same HPTLC plate, biochemical profiling four different (bio)assays elucidation structures known unidentified compounds. five AChE inhibitors, salvianolic acid B (SAB), lithiospermic (LSA) rosmarinic (RA) as well cryptotanshinone (CT) 15,16-dihydrotanshinone I (DHTI) confirmed, but also inhibitors observed. In extracts, SAB, LSA RA exhibited free radical scavenging 2,2-diphenyl-1-picrylhydrazyl assay. CT, DHTI some found against Gram-positive Bacillus subtilis Gram-negative Aliivibrio fischeri (LOD 12 ng/band 5 ng/band DHTI). For first time, most multipotent compound B. subtilis, A. fingerprints extract identified co-eluted band 1,2-dihydrotanshinone methylenetanshinquinone ratio 2:1.