Variability and evolution of Kaposi's sarcoma-associated herpesvirus in Europe and Africa

摘要: OBJECTIVE: To study the evolution of Kaposi's sarcoma-associated herpesvirus (KSHV) or human type 8 in Europe and Africa. DESIGN AND METHODS: PCR sequence analysis variable viral membrane glycoprotein gene K1 58 tumour peripheral blood samples from patients with AIDS-related sarcoma (KS), 'classic' (HIV-negative) KS, transplant Multicentric Castleman's Disease, other lymphoproliferative disorders, healthy KSHV-infected individuals UK, Denmark, Sweden, Italy, Spain, Iceland, The Faroe Islands, Greece, Gambia Uganda. RESULTS: Three major groups sequences were found: A, B C, as defined previously. has evolved, both within between these three groups, under positive selection. KSHV group strains predominate Africa are more distant A found Europe, than C each other. Within two subgroups, C' C", can be identified. Subgroup C" is closely related to a region protein appears phylogenetically close branchpoint C. Group currently HIV-1-infected -uninfected Europeans, already present before start AIDS epidemic. We some examples Italy but general did not cluster geographically. CONCLUSION: KSHV East West those Europe. related, adopting an intermediate phylogenetic position. In may have been disseminated at least several decades ago. Variability driven by selection does correlate different KSHV-related pathologies geographic regions where clinically aggressive HIV-negative KS ('endemic' KS) common.