Agarose-assisted micro-contact printing for high-quality biomolecular micro-patterns.

摘要: Micro-contact printing has been developed to print biomolecules, such as cell adhesive molecules, proteins, or DNAs, on a substrate, which can serve experimental platforms for investigating biological issues and engineering biosensors. Despite the popularity of this method, it technically challenging use conventional stamp made hydrophobic polydimethoxysilane (PDMS) elastomer that often requires surface treatments facilitate inking stamping biomolecules. In work, we proposed new modification method PDMS using agarose hydrogel demonstrated applications design micro-patterned substrates with By simple bench-top dip-coating commercial syringe pump steadily pull out from boiled solution, coated an layer stamp. It consequentially enhanced transferability ink molecules target substrate uniformity printed patterns compared traditional methods treating surfactant coating temporary oxidation air plasma. addition, microstamping was also used produce proteins preservation bioactivity, could guide neuronal growth. Thus, applicability interface designs biochips