Microcontact printing of polydopamine on thermally expandable hydrogels for controlled cell adhesion and delivery of geometrically defined microtissues

摘要: Abstract Scaffold-free harvest of microtissue with a defined structure has received great deal interest in cell-based assay and regenerative medicine. In this study, we developed thermally expandable hydrogels spatially controlled cell adhesive patterns for rapid geometrically microtissue. We patterned polydopamine (PD) on to the hydrogel via microcontact printing (μCP), linear shapes widths 50, 100 200 μm. The facilitated formation strip-like human dermal fibroblasts (HDFBs). It was possible translocate microtissues 61.4 ± 14.7, 104.3 ± 15.6, 186.6 ± 22.3 μm from glass substrates by conformal contact upon expansion response temperature change 37 4 °C, preserving high viability, extracellular matrix, junction proteins. Microtissues were readily translocated vivo subcutaneous tissue mouse. further utilized as simple model monitoring contraction ROCK1 inhibitor. Collectively, micro-sized patterning PD μCP holds promise development harvesting systems that can be employed ex therapy. Statement significance Harvest artificial cellular arrangement independently external materials been widely studied scaffold-free system anisotropic width exploiting cell-adhesive formed printing. Cultured strips rapidly delivered various targets ranging flat coverglass mice thermal at 4 °C 10 min. These drug screening responding inhibitor, which imply its versatile applicability.