Lineage infidelity following exposure of T lymphoblasts (MOLT-3 cells) to 5-azacytidine

摘要: The appearance on single leukemic blast cells of markers at least two different lineages has been termed lineage infidelity. MOLT-3 cells, a continuous line human T lymphoblasts, express cell as defined immunologically with monoclonal antibodies. Following exposure to 5-azacytidine, other markers, usually associated non-T lineages, appeared transiently. A stable clone infidelity was obtained by selection from colonies expressing novel markers. Marker expression followed reproducible kinetics during growth. 5-Azacytidine-treated subclones may be useful models in the study and gene expression.