Rejection of skin allografts by indirect allorecognition of donor class I major histocompatibility complex peptides.

摘要: LEW (RT1l) rats were immunized with peptides corresponding to the alpha helical region of 1 domain (peptide 1), beta sheet 2 2), and 3) RT1-Aav1 classical class I molecule DA (RT1av1) strain. The immunizations without carriers, objective was prime indirect allorecognition influencing direct recognition molecule. mounted strong primary secondary antibody responses 3, but only weak peptide 2. None antipeptide antibodies crossreacted intact molecules. immunization also resulted in antigen-presenting cell-dependent, CD4+ T cell proliferative responses, which very against weakest or most effectively both 3 together, showed accelerated rejection skin allografts. This effect not observed In response allograft, peptide-immunized markedly kinetics production These data demonstrate that can play an important role allograft have implications for understanding its regulation.