The effect of microbial-nutrient interaction on the immune system of young chicks after early probiotic and organic acid administration.

摘要: The combined effects of probiotics (Lactobacillus acidophilus, Lactobacillus casei, Streptococcus faecium, and Saccharomyces cerevisiae) organic acids (sorbic citric acid) on intestinal morphology expression immune-related genes were investigated. One-day-old chicks randomly assigned to 1 3 groups: birds not receiving probiotic or (control; T1), an oral combination (1 g/L in water) 10(8) CFU/g each the aforementioned (1% sorbic acid 0.2% for 7 (T2) 14 d (T3). Each group was divided into 5 replicate pens 20 each, from pen) killed 11 22. Intestinal sections collected histological assessment, reverse transcriptase-PCR analysis used assess defensin cathelicidins expression. Quantitative real-time PCR toll-like receptors (TLR) cytokine Duodenal villus height greater T2 T3 at (P ≤ 0.036) 22 0.015) compared with T1. At 11, duodenal goblet cell/unit area less T3, whereas it T1 jejunum = 0.009). Ileal < 0.001). Avian beta-defensin-3 expressed all tissues except bursa TLR-2 down regulated cecal tonsil 0.020 0.003, respectively). Expression IL-12p35 ileum 0.030 0.012, Reduced INF-γ observed 0.047). IL-6 IL-10 interferon-gamma (INF-γ) expressions 0.047) than In conclusion, supplementation resulted inconsistent gut associated responses, avian beta-defensins supplementation. Birds supplemented showing similar responses TLR-2, IL-12p35, IFN-γ those indicates that shorter periods might be enough elicit beneficial responses.