Age-related changes in ADMA–DDAH–NO pathway in rat liver subjected to partial ischemia followed by global reperfusion

摘要: Abstract Background Liver function is affected during ischemia/reperfusion (IR). We evaluated the effect of aging process on selected parameters determining NO level in rat liver subjected to IR. Methods The animals were divided into C-2 and IR-2 group young rats (2–4 months old) C-12 IR-12 older (12–14 months old). Livers belonging partial ischemia (60 min) reperfusion (4 h). Blood samples obtained after surgeries estimate activity aminotransferases, as well just before (15, 120, 240 min) concentration arginine (Arg) its derivatives: asymmetric symmetric dimethylarginine (ADMA, SDMA). After IR, dimethylaminohydrolase (DDAH) protein inducible nitric oxide synthase (iNOS) measured homogenates. Results In ADMA increased most between 15 120 min was highest all groups (0.72 ± 0.2 μmol/l). decreased significantly lower compared other at 15 min (0.42 ± 0.2 μmol/l) 120 (0.52 ± 0.1 μmol/l) 240 min (0.38 ± 0.1 μmol/l) reperfusion. Only SDMA (0.75 ± 0.9 μmol/l) (1.0 ± 1.2 μmol/l) At beginning surgery Arg higher (C-2: 102.1 ± 35.7 μmol/l; IR-2: 114.63 ± 28.9 μmol/l) than ones (C-12: 41.88 ± 44.7 μmol/l; IR-12: 28.64 ± 30.6 μmol/l). (77.41 ± 37.5 μmol/l) Arg/ADMA (A/A) ratio (138.03 ± 62.8 μmol/l) ischemic (Arg: 47.17 ± 31.7 μmol/l; A/A: 88.28 ± 66.2 μmol/l) 43.87 ± 21.9 μmol/l; 118.02 ± 106.3 μmol/l). (11.4 ± 12.0 μmol/l) A/A (16.11 ± 16.2 μmol/l) next phase levels those low, comparably IR-12. As a result decrease DDAH an increase iNOS observed only rats. Conclusions found that non-ischemic may be by process. Under IR conditions, important changes DDAH–ADMA–NO pathway livers.