Method of producing insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) with correct folding and disulfide bonding
作者： Yasushi Ogawa , Andreas Sommer , Peggy Tao
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摘要: The present invention provides a novel method for refolding insulin-like growth factor I (IGF-I) and binding protein 3(IGFBP-3). involves mixing of IGF-I IGFBP-3 together in cofolding reaction. inventive results substantially higher yields correctly folded both molecules alters the kinetics refolding. includes production IGF-I, IGFBP-3, and/or IGF-I/IGFBP-3 complex.
glgoo.top参考文章(23)
Sophia Hober, Anna Hansson, Mathias Uhlen, Bjoern Nilsson, Folding of Insulin-like Growth Factor I Is Thermodynamically Controlled by Insulin-like Growth Factor Binding Protein Biochemistry. ,vol. 33, pp. 6758- 6761 ,(1994) , 10.1021/BI00188A003
Sophia Hober, Goeran Forsberg, Gunnar Palm, Maris Hartmanis, Bjoern Nilsson, Disulfide exchange folding of insulin-like growth factor I Biochemistry. ,vol. 31, pp. 1749- 1756 ,(1992) , 10.1021/BI00121A024
F. Raschdorf, R. Dahinden, W. Maerki, W. J. Richter, J. P. Merryweather, Location of disulphide bonds in human insulin‐like growth factors (IGFs) synthesized by recombinant DNA technology Journal of Mass Spectrometry. ,vol. 16, pp. 3- 8 ,(1988) , 10.1002/BMS.1200160102
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)
John R. Ogez, Stuart E. Builder, Purification and activity assurance of precipitated heterologous proteins ,(1984)
Larry B. Tsai, James A. Miller, Philip K. Hsieh, Production of biologically active insulin-like growth factor i from high expression host cell systems ,(1990)
Sophia Elisabeth Hober, Bjorn Nilsson, Use of igf-bp for refolding of igf ,(1994)
Judy Y. Chang, Nancy C. McFarland, James R. Swartz, Method for refolding insoluble, misfolded insulin-like growth factor-1 into an active conformation ,(1991)
C H Squires, J Childs, S P Eisenberg, P J Polverini, A Sommer, Production and characterization of human basic fibroblast growth factor from Escherichia coli. Journal of Biological Chemistry. ,vol. 263, pp. 16297- 16302 ,(1988) , 10.1016/S0021-9258(18)37592-6
C.C. Liu, H.I. Miller, W.J. Kohr, J.I. Silber, Purification of a ubiquitin protein peptidase from yeast with efficient in vitro assays. Journal of Biological Chemistry. ,vol. 264, pp. 20331- 20338 ,(1989) , 10.1016/S0021-9258(19)47067-1