Production and characterization of human basic fibroblast growth factor from Escherichia coli.
作者： C H Squires , J Childs , S P Eisenberg , P J Polverini , A Sommer
DOI: 10.1016/S0021-9258(18)37592-6
关键词: Molecular biology 、 Basic fibroblast growth factor 、 Biology 、 Fibroblast growth factor receptor 4 、 Fibroblast growth factor 、 FGF10 、 T7 RNA polymerase 、 Fibroblast growth factor receptor 3 、 Fibroblast growth factor receptor 、 Fibroblast growth factor receptor 2
摘要: The cDNA sequence coding for human basic fibroblast growth factor (bFGF) has been cloned downstream of a transcription promoter recognized by the bacteriophage T7 RNA polymerase. Initiation translation at fgf gene coupled to upstream fragment phi 10 gene. Expression in this system can lead an accumulation approximately 40 mg/liter/A600 unit bFGF. This material be purified close homogeneity from soluble protein extract on heparin-Sepharose column. bFGF so obtained shown have bioactivity indistinguishable placental mitogenicity, synthesis plasminogen activator, and angiogenesis assays.
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