Sheathless interface to match flow rate of capillary electrophoresis with electrospray mass spectrometry using regular-sized capillary.

摘要: Rationale The flow rate match has been a great challenge when coupling capillary electrophoresis (CE) with electrospray ionization mass spectrometry (ESI-MS). Conventional CE-ESI-MS interfaces used liquid sheath flow, narrowed or additional pressure to meet this requirement; sacrifice of either inner diameter (i.d.) separation efficiency is often inevitable. Thus, regular-sized capillary-based sheathless interface would be attractive for in CE-MS. Methods CE-MS was achieved by CE induced (iESI) which stimulated the fact that iESI could both achieve down 0.2 μL/min and retain efficiency. CE-iESI-MS completed an intact capillary, outside outlet end metal electrode attached application alternating current (ac) high voltage (HV). Results feasibility demonstrated through stable total ion chromatograms obtained continuous infusion tripropylamine capillaries. Tripropylamine atenolol were separated detected successfully phosphate buffer solution (PBS) using 50 75 μm i.d. capillary. Furthermore, new showed better signal-to-noise (S/N) 3 7 times enhancement compared another one analyzing mixture proline NH4 OAc buffer. In addition, reproducibility gave satisfactory results relative standard deviation (RSD) retention time range between 1% 3%. Conclusions novel introduced here conventional electroosmotic (EOF) also preserve sensitivity This newly developed effective different buffers, PBS OAc, without any additives such as methanol acetic acid. Hence, we believe operated other nonvolatile volatile buffers. Copyright © 2016 John Wiley & Sons, Ltd.