Assessment of the endocrine-disrupting effects of diethyl phosphate, a nonspecific metabolite of organophosphorus pesticides, by in vivo and in silico approaches.

摘要: Organophosphorus pesticides (OPs) remain one of the most commonly used pesticides, and their detection rates residues in agricultural products, foods environmental samples have been underestimated. Humans organisms are at high risk exposure to OPs. Most OPs can be degraded metabolized into dialkyl phosphates (DAPs) environment, present urine as biomarkers for OPs, which diethyl phosphate (DEP) is a high-exposure metabolite. Epidemiological cohort studies found that DAPs associated with endocrine hormone disorders, especially sex disorders thyroid but there has no direct causal evidence support these findings. Our study explored effects chronic DEP on hormones related metabolic indicators adult male rats actual doses reached human body. The results showed could cause thyroid-related serum rats, causing symptoms hyperthyroidism also lead abnormal expression hormone-related genes rat liver. However, did not seem affect levels, spermatogenesis or sperm quality rats. molecular interactions between enzymes/proteins were investigated by docking dynamics methods silico. It was strongly interact biosynthesis, blood transport, receptor binding metabolism-related enzymes/proteins, interfering production signal regulation hormones. In vivo silico experiments might potential hormone-disrupting chemical, therefore, we need more cautious rigorous regarding organophosphorus chemical exposure.