Effect of the Isocoumarin Paepalantine on the Luminol and Lucigenin Amplified Chemiluminescence of Rat Neutrophils
作者： Rodrigo Rezende Kitagawa , Maria Stella Gonçalves Raddi , Najeh Maissar Khalil , Wagner Vilegas , Luiz Marcos da FONSECA
DOI: 10.1248/BPB.26.905
关键词:
摘要: Paepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naphto(2,3c)pyran-1-one), a natural isocoumarin isolated from the capitula of Paepalanthus bromelioides (Eriocaulaceae), was assessed for its effect on respiratory burst (zymosan-stimulated luminol-enhanced chemiluminescence and PMA-stimulated lucigenin-enhanced chemiluminescence) polymorphonuclear neutrophils in vitro. Special attention devoted to establishing IC50 neutrophils. able decrease luminol lucigenin chemiluminescence, reflecting an inhibitory burst, with ED50 0.44±0.05 0.84±0.15 μg/ml, respectively. A cell-free system performed paepalantine myeloperoxidase/H2O2 myeloperoxidase/H2O2/Cl− systems. inhibited oxidation both This inhibition related interaction paepalantine-myeloperoxidase scavenger HOCl.
sci-hub.se 参考文章(20)
Frank A. Barile, Introduction to In Vitro Cytotoxicology: Mechanisms and Methods ,(2019)
Constanze Bergt, Gunther Marsche, Ute Panzenboeck, Jay W. Heinecke, Ernst Malle, Wolfgang Sattler, Human neutrophils employ the myeloperoxidase/hydrogen peroxide/chloride system to oxidatively damage apolipoprotein A-I. FEBS Journal. ,vol. 268, pp. 3523- 3531 ,(2001) , 10.1046/J.1432-1327.2001.02253.X
D A Bass, P S Shirley, M J Thomas, M S Cohen, G D Long, L R DeChatelet, F W Henderson, Mechanism of the luminol-dependent chemiluminescence of human neutrophils. Journal of Immunology. ,vol. 129, pp. 1589- 1593 ,(1982)
Wagner Vilegas, Nídia F. Roque, Antonio Salatino, Astréa M. Giesbrecht, Solange Davino, Isocoumarin from Paepalanthus bromelioides. Phytochemistry. ,vol. 29, pp. 2299- 2301 ,(1990) , 10.1016/0031-9422(90)83056-7
Claes Dahlgren, Anna Karlsson, Respiratory burst in human neutrophils. Journal of Immunological Methods. ,vol. 232, pp. 3- 14 ,(1999) , 10.1016/S0022-1759(99)00146-5
Anne E.F.Van Nerom, Richard Ducatelle, Freddy Haesebrouck, Oxygen radicals and nitric oxide production by turkey respiratory macrophages Developmental and Comparative Immunology. ,vol. 22, pp. 407- 416 ,(1998) , 10.1016/S0145-305X(98)00019-6
Arti Arora, Muraleedharan G. Nair, Gale M. Strasburg, Structure-activity relationships for antioxidant activities of a series of flavonoids in a liposomal system. Free Radical Biology and Medicine. ,vol. 24, pp. 1355- 1363 ,(1998) , 10.1016/S0891-5849(97)00458-9
Dae-Sig Jang, Bo-Seong Kang, Shi Yong Ryu, IL-Moo Chang, Kyung Rak Min, Youngsoo Kim, Inhibitory effects of resveratrol analogs on unopsonized zymosan-induced oxygen radical production. Biochemical Pharmacology. ,vol. 57, pp. 705- 712 ,(1999) , 10.1016/S0006-2952(98)00350-5
Bernard M. Babior, Phagocytes and oxidative stress The American Journal of Medicine. ,vol. 109, pp. 33- 44 ,(2000) , 10.1016/S0002-9343(00)00481-2
LIBRADA M. CAÑEDO, JOSÉ L. FERNÁNDEZ PUENTES, JULIA PÉREZ BAZ, CRISTINA ACEBAL, FERNANDO DE LA CALLE, DOLORES GARCÍA GRÁVALOS, TERESA GARCÍA DE QUESADA, PM-94128, a new isocoumarin antitumor agent produced by a marine bacterium The Journal of Antibiotics. ,vol. 50, pp. 175- 176 ,(1997) , 10.7164/ANTIBIOTICS.50.175