A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25

摘要: Botulinum neurotoxin type-A (BoNT/A), as onabotulinumtoxinA, is approved globally for 11 major therapeutic and cosmetic indications. While the mechanism of action BoNT/A at presynaptic nerve terminal has been established, questions remain regarding intracellular trafficking patterns overall fate toxin. Resolving these partly depends on ability to detect BoNT/A’s location, distribution, movement within a cell. Due high potency extremely low concentrations neurons, an alternative approach employed. This involves utilizing specific antibodies against BoNT/A-cleaved SNAP25 substrate (SNAP25197) track enzymatic activity toxin cells. Using our highly mouse monoclonal antibody (mAb) SNAP25197, we generated human murine recombinant versions (rMAb) using backbone immunoglobulins. In this study, validated specificity anti-SNAP25197 rMAbs in several different assays performed side-by-side comparisons commercially-available in-house SNAP25. Our were SNAP25197 all BoNT/A-treated tissues, showing no cross-reactivity with full-length was not case other reportedly SNAP25197-selective antibodies, which selective some, but assays. The described herein represent effective new tools detecting