Diphenyl diselenide protects against methylmercury-induced inhibition of thioredoxin reductase and glutathione peroxidase in human neuroblastoma cells: a comparison with ebselen.

摘要: Exposure to methylmercury (MeHg), an important environmental toxicant, may lead serious health risks, damaging various organs and predominantly affecting the brain function. The toxicity of MeHg can be related inhibition selenoenzymes, such as glutathione peroxidase (GPx) thioredoxin reductase (TrxR). Experimental studies have shown that selenocompounds play role cellular detoxifiers protective agents against harmful effects mercury. present study investigated mechanisms by which diphenyl diselenide [(PhSe)2 ] ebselen interfered with interaction mercury (MeHg) selenoenzymes (TrxR GPx) in vitro experimental model cultured human neuroblastoma cells (SH-SY5Y). Our results established (PhSe)2 increased activity expression TrxR. In contrast, inhibited TrxR even at low doses (0.5 μm). Coexposure showed a effect on both When selenoenzyme GPx was evaluated, did not alter its or significantly, whereas (from 1 Among only significantly protected activity. Taken together, these indicate potential use for toxicity. Furthermore, first time, we demonstrated caused more pronounced upregulation than cells, likely reflecting molecular mechanism involved antioxidant properties this compound. Copyright © 2017 John Wiley & Sons, Ltd.