Triplex-hybridizing bioconjugated gold nanoparticles for specific Y-chromosome sequence targeting of bull spermatozoa

摘要: Gold nanoparticles (AuNPs) are widely used in biomedical applications for drug targeting and bioimaging. This often neccesitates their functionalization with biomolecules carrying a defined biological function, yielding gold nanoparticle bioconjugates. The utilization of triplex-forming oligonucleotides (TFOs) as ligands gives access to nanoconjugates tools specific DNA-related nanotargeting via triplex hybridization. Since hybridization nanobioconjugates has date not been shown on biologically relevant samples, sex-specific sperm marking may be an appropriate model system demonstrate the opportunities this method vitro. In study, we focused labeling repetitive target sites enriched bovine Y-chromosome using forming oligonucleotides. First, functionality locked nucleic acid (LNA) sequence was confirmed free DNA demembranated heads. Thereafter, influence AuNPs spectrophotometrically analyzed employing synthetic dsDNA, genomic Results from SPR-peak shift indicate that TFO-AuNP hybridize gDNA qualitative significant manner. These results confirm successful well establishment use suitable tool sex-selective