Application of Time-Resolved Fluorescence Spectroscopy to Studies of DNA-Protein Interactions and RNA Folding
作者： D. P. Millar
DOI: 10.1007/978-3-642-56853-4_20
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摘要: In the life sciences, fluorescence spectroscopy has been widely used as a tool to investigate structure and dynamics of proteins [1,2] The widespread use spectroscopic techniques stems from occurrence naturally fluorescent amino acids tryptophan tyrosine in many A number have just single or residue, considerably simplifying interpretation data Moreover, through site-directed mutagenesis procedures, it is relatively straightforward engineer for studies by incorporating residues at desired positions removing all but one residue protein interest.
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