Cell differentiation features in embryos resulting from interphylum nuclear transplantation: Echinoderm nucleus to ascidian zygote cytoplasm

摘要: Abstract When an echinoderm nucleus was transplanted into ascidian zygote cytoplast there developmental cooperation at the cellular level between and cytoplasm of these normally nonhybridizable species. A blastula stage from sand dollar Echinarachnius parma injected activated but nonnucleate egg fragment Ciona intestinalis . During culture, some “hybrid” embryos displayed ultrastructural evidence differentiation. Two recognizable features were (1) extracellular matrix components, (2) neural cell characteristics, including elaboration associated cilia. Nonnucleate fragments alone, such with seawater or punctured by glass needle, did not develop organized subcellular structures. Morphologic expressions resulting nuclear transplantations two phyla (Echinodermata Chordata) seemingly indicate functional interactions a gene regulatory level. Creation nuclear-cytoplasmic hybrids suggests thereby means exploring nature cytoplasmic agents in that appear to determine expression related histospecific differentiation products.