High urea and NaCl carbonylate proteins in renal cells in culture and in vivo, and high urea causes 8-oxoguanine lesions in their DNA

摘要: Urea and NaCl are elevated in the renal inner medulla. We now find that a high concentration of urea or increases reactive oxygen species (ROS) mouse medullary (mIMCD3) cells culture. Previously, NaCl, but not urea, was found to cause DNA double-strand breaks. tested whether causes oxidative damage cellular proteins. mIMCD3 cell single-strand breaks 8-oxoguanine lesions. High does detectable also greatly carbonylation proteins cells. Carbonylation occurs within 5 min with as little mM normal plasma level. It is raised over range uremia. A raffinose level ROS carbonylation. sorbitol glycerol levels do increase Carbonyl content medullas where interstitial concentrations normally high. There, numerous carbonylated, both collecting ducts thin limbs. Conclusions: (i) Oxidative stress, associated lesions DNA. (ii) carbonylates vivo. (iii) In carbonylation, uremic concentration, indicating can contribute directly