Evaluation of DNA Damage and Oxidative Stress Inductions by Excessive Medical Intake of Saline in Mice Bone Marrow Cells

摘要: Sodium chloride (NaCl) is widely used in various industrial, medical and food applications that increasing human exposure to it by excessive amount. However, almost all searches focused on its nephrotoxicity adaptation of kidney cells high salinity only few scientific attentions were concerned genotoxicity. As the vast majority drugs especially anticancer diluted saline injected i.p. route. Hence, oxidative DNA damage induction intake mice bone marrow was investigated. Mice with NaCl solution at fixed dose level (10 ml/kg b.w. /2 days) both normal (0.9%) (3% & 5%) for six times sacrificed 24 hour after last injection. Micronucleus comet assays gel pulsed field electrophoresis evaluate possible genotoxicity chromosomal levels. Also, some biochemical markers stress assessed shed more light mechanism action. evidenced statistical significant elevations micronucleated polychromatic erythrocytes tail moment a dose-dependent manner. This further appearance fragmentized smeared agarose gel. Moreover, caused elevation malondialdehyde decreases reduced glutathione superoxide dismutase peroxidase activities dependent In conclusions we can say that  even though concentration (0.9%). reactive oxygen species generation ability observed lipid peroxidation marker reductions antioxidant defense system. Therefore, it’s recommended decrease or use dilutions due dangerous genotoxic effects.