Silver Methods for the Impregnation of Degenerating Axoplasm

摘要: The reduced silver methods were introduced in the beginning of this century (Ramon y Cajal, 1904; Bielschowsky, 1904), and they initially appreciated primarily for their remarkable capacity to reveal normal histology fiber architecture brain. Unfortunately, attempts trace specific systems using these generally unsuccessful, because bundles from a selected neuronal cell group could not be followed with any certainty through complex meshwork axons arising other parts nervous system. In 1930s, neuroanatomists (e.g., Hoff, 1932a,b; Glees Le Gros Clark, 1941; Glees, 1946) discovered that stains identify some stage Wallerian degeneration* utilized “mark” system interest, thus facilitating its recognition as it meandered Thus, an area subjected experimental lesion, and, after appropriate survival interval, animal sacrificed, brain processed by method degenerating en route destination. Although usually distinguished ones such preparations, evaluating histological material was tedious, results often inaccurate or incomplete. A major breakthrough occurred when Nauta his colleagues (Nauta, 1950; Gygax, 1951, 1954; Ryna, 1952) first developed stain selective neural elements.