Expressed sequence tags and proteomics of antennae from the tortricid moth, Epiphyas postvittana.
作者： M. D. Jordan , D. Stanley , S. D. G. Marshall , D. De Silva , R. N. Crowhurst
DOI: 10.1111/J.1365-2583.2008.00812.X
关键词:
摘要: Genomic and proteomic analyses of the antennae light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae) were undertaken to identify genes proteins potentially involved in odorant pheromone binding turnover. An EST approach yielded 5739 sequences, comprising 808 contigs 1545 singletons. InterPro Blast revealed members families implicated (PBPs, GOBPs, ABPXs CSPs) turnover (CXEs, GSTs, CYPs). Of three (PBPs) identified, two more highly expressed at RNA protein levels adult male (EpPBP1, EpPBP3), while a third was female (EpPBP2). To detection sex-specific signals, differential 2D gel electrophoresis (pH 5-8) followed by mass spectrometry conducted on antennal from males versus females. Identified male-biased included protein, porin, short chain dehydrogenase/reductase, member takeout family.
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