RBM5 reduces small cell lung cancer growth, increases cisplatin sensitivity and regulates key transformation-associated pathways

摘要: Small cell lung cancer (SCLC) is the most aggressive type of cancer, with almost 95% patients succumbing to disease. Although RBM5, a tumor suppressor gene, downregulated in majority cancers, its role SCLC unknown. Using GLC20 line, which has homozygous deletion encompassing RBM5 gene locus, we established stable expressing sublines and investigated effects re-expression. Transcriptome target identification studies determined that directly regulates cycle apoptosis cells, as well significantly downregulates other important transformation-associated pathways such angiogenesis adhesion. RNA sequencing paired non-tumor patient specimens showed decreased expression tumors, alterations same were altered cells sublines. Functional confirmed slows line growth, increases sensitivity chemotherapy drug cisplatin. Overall, our work demonstrates importance non-transformed state consequences development progression.