Quantification and mechanisms of oleic acid-induced steatosis in HepG2 cells

摘要: Developing a quantifiable in vitro model of steatosis is critical understanding the pathogenesis nonalcoholic fatty liver disease (NAFLD) and searchingfor effective therapies. Using an ORO-based colorimetric measurement, we developed convenient assay to qualify degree OA-induced HepG2 cells. We demonstrated that absence exogenous inflammatory mediators, was associated with increased production secretion tumor necrosis factor alpha decreased expression peroxisome proliferators-activated receptor α also lipid peroxidation, apoptosis, but proliferation these The peroxidation related SOD-1, free radical scavenger enzyme; while apoptosis active caspase-9. mediated by p27 unchanged alanine transaminase (ALT) level culture medium, indicating alters cell cycle progression without direct toxicity In conclusion, present study accurately quantifies results series pathophysilogical changes cells, pathogenic roles hepatocytes NAFLD.