Estudio genético y funcional de NR5A2, un factor de transcripción ligado a obesidad y diabetes
作者： Alfonso Bolado Carrancio
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摘要: RESUMEN: NR5A2 es un receptor nuclear huerfano asociado al metabolismo del colesterol, sus derivados y diferenciacion de tejidos, que recientemente se ha implicado en el mantenimiento la homeostasis glucosa. Ademas, estudio asociacion previo, SNP promotor mostro con IMC. Partiendo esto, realizamos posible implicacion desarrollo enfermedades metabolicas complejas, como obesidad o diabetes. Realizamos secuenciado RNA para localizar los genes energetico regulados por empleamos modelos lineas celulares higado (HepG2) mcusculo (C2C12), expresion silenciada, sobreexpresada activada mediante agonista sintetico. Nuestros resultados muestran reduce captacion glucosa sintesis TAGs HepG2 favorece consumo C2C12, disminuyendo acidos grasos. Tomando estos resultados, podria actuar agente protector contra las patologias asociadas a resistencia insulina.
unirioja.es参考文章(140)
G. Bunone, P. A. Briand, R. J. Miksicek, D. Picard, Activation of the unliganded estrogen receptor by EGF involves the MAP kinase pathway and direct phosphorylation. The EMBO Journal. ,vol. 15, pp. 2174- 2183 ,(1996) , 10.1002/J.1460-2075.1996.TB00571.X
David M. Heery, Eric Kalkhoven, Susan Hoare, Malcolm G. Parker, A signature motif in transcriptional co-activators mediates binding to nuclear receptors Nature. ,vol. 387, pp. 733- 736 ,(1997) , 10.1038/42750
Christian Vaisse, Karine Clement, Bernard Guy-Grand, Philippe Froguel, A frameshift mutation in human MC4R is associated with a dominant form of obesity Nature Genetics. ,vol. 20, pp. 113- 114 ,(1998) , 10.1038/2407
Marco De Gobbi, Vip Viprakasit, Jim R Hughes, Chris Fisher, Veronica J Buckle, Helena Ayyub, Richard J Gibbons, Douglas Vernimmen, Yuko Yoshinaga, Pieter De Jong, Jan-Fang Cheng, Edward M Rubin, William G Wood, Don Bowden, Douglas R Higgs, None, A Regulatory SNP Causes a Human Genetic Disease by Creating a New Transcriptional Promoter Science. ,vol. 312, pp. 1215- 1217 ,(2006) , 10.1126/SCIENCE.1126431
Wei Cui, Stephen L Chen, Ke-Qin Hu, None, Quantification and mechanisms of oleic acid-induced steatosis in HepG2 cells American Journal of Translational Research. ,vol. 2, pp. 95- 104 ,(2010)
Peter G. Kopelman, Obesity as a medical problem Nature. ,vol. 404, pp. 635- 643 ,(2000) , 10.1038/35007508
Dali Chen, Abhimanyu Garg, Monogenic disorders of obesity and body fat distribution Journal of Lipid Research. ,vol. 40, pp. 1735- 1746 ,(1999) , 10.1016/S0022-2275(20)34890-2
Yiqiang Zhao, Wyatt T. Clark, Matthew Mort, David N. Cooper, Predrag Radivojac, Sean D. Mooney, Prediction of functional regulatory SNPs in monogenic and complex disease Human Mutation. ,vol. 32, pp. 1183- 1190 ,(2011) , 10.1002/HUMU.21559
Cédric Le May, Thierry Pineau, Karine Bigot, Claude Kohl, Jean Girard, Jean-Paul Pégorier, Reduced hepatic fatty acid oxidation in fasting PPARα null mice is due to impaired mitochondrial hydroxymethylglutaryl-CoA synthase gene expression FEBS Letters. ,vol. 475, pp. 163- 166 ,(2000) , 10.1016/S0014-5793(00)01648-3
E. Matthew Morris, Grace M. E. Meers, Frank W. Booth, Kevin L. Fritsche, Christopher D. Hardin, John P. Thyfault, Jamal A. Ibdah, PGC-1α overexpression results in increased hepatic fatty acid oxidation with reduced triacylglycerol accumulation and secretion American Journal of Physiology-gastrointestinal and Liver Physiology. ,vol. 303, ,(2012) , 10.1152/AJPGI.00169.2012