Primer probe combination and fluorescence quantitative polymerase chain reaction (PCR) kit for simultaneous detection of three types of cryptococcus
作者： Fang Yuan , Ouyang Yun , Lyu Huoyang
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摘要: The invention relates to a primer probe combination and fluorescence quantitative polymerase chain reaction (PCR) kit for simultaneous detection of three types cryptococcus, belonging the technical field in-vitro molecular pathogenic microorganisms. provides cryptococcus based on PCR method. comprise neoformans, gattii laurentii; primers an upstream downstream primer, wherein nucleotide sequence is shown in SEQ ID No.1, No.2; substance which modified with fluorophore at 5'end quenching group 3'end No.3. provided by can realize laurentii, high specificity sensitivity.
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