Downregulation of XIAP expression induces apoptosis and enhances chemotherapeutic sensitivity in human gastric cancer cells.

摘要: X-linked inhibitor of apoptosis (XIAP) is the most potent member protein (IAP) gene family in terms its ability to inhibit caspases and suppress apoptosis. Recent evidence has suggested that XIAP a key determinant chemoresistance cancer cells. To explore novel approach for ameliorating chemotherapy gastric cancer, antisense expression vector was constructed transferred into cell lines, MKN-45 (wild-type p53) MKN-28 (mutant-type p53). This transfer resulted significant downregulation expression, decreased vitro viabilities, induced In cells, inactive caspase-3 precursors were cleaved active subunits (p20 p17) during by XIAP. The inhibitory effects cisplatin mitomycin C on growth downregulated cells significantly enhanced. addition, this process occurred only wild-type p53 (MKN-45), but not mutant-type (MKN-28) data presented suggest via RNA can lead vitro, correlating with cellular status activation caspase-3. finding could potential strategy improving efficiency therapies cancer.