Glutamate dehydrogenase is essential to sustain neuronal oxidative energy metabolism during stimulation

摘要: The enzyme glutamate dehydrogenase (GDH; Glud1) catalyzes the (reversible) oxidative deamination of to α-ketoglutarate accompanied by a reduction NAD+ NADH. GDH connects amino acid, carbohydrate, neurotransmitter and energy metabolism. Glutamine is precursor used neurons sustain pool glutamate, but glutamine also vividly oxidized for support This study investigates role in neuronal metabolism employing Cns- Glud1-/- mouse, lacking brain (GDH KO) metabolic mapping using 13C-labelled glucose. We observed severely reduced during glucose deprivation synaptosomes cultured not expressing GDH. In contrast, presence glucose, was affected lack expression. Respiration fuelled significantly lower mitochondria from KO mice were able increase their respiration upon an elevated demand. respiratory capacity underscore importance particularly demand, it may reflect large allosteric activation ADP.