Soluble factors in tolerance and contact sensitivity to 2,4-dinitrofluorobenzene in miceIV. Characterization of migration inhibition factor-producing lymphocytes and genetic requirements for activation

摘要: The production of migration inhibition factor (MIF) in vitro by lymph node cells from mice with contact sensitivity to 2,4-dinitrofluorobenzene (DNFB) was investigated. MIF activity cell-free culture supernatants measured using a micro, indirect "hanging-drop" assay system. We found that DNFB-sensitized are stimulated produce co-culture DNP-labeled spleen or splenic adherent cells. stimulation quantitatively antigen-specific, as TNP-spleen TNP-splenic induced only low levels activity. Pretreating the immune different antisera plus complement, before addition DNP-spleen cells, showed is dependent on Ia- T Additional experiments order for be stimulated, homology at I-A subregion major histocompatibility complex between and required. Collectively, these results correlate our previous finding transfer mediated indicate both tests, i.e., vivo vitro, measuring effector functions same cell subset.