Stimulation of Mouse Migration Inhibitory Factor (MIF) Production from MSV-Immune Lymphocytes by Soluble Tumor-Associated Antigen: Requirement for Histocompatible Macrophages

摘要: Spleen cells from C57BL/6 mice immunized with murine sarcoma virus (MSV) are capable of producing migration inhibition factor (MIF) in response to stimulation a specific tumor-associated antigen prepared by solubilization 3 M KCl. We have previously demonstrated that this is T cell dependent. Further investigations into the effector involved production MIF revealed spleen MSV cannot produce when stimulated tumor extract if population has been depleted macrophages. However, can be restored adding nonimmune syngeneic macrophages but not allogeneic The inability provide function was due their increased suppressor activity since mixing experiments they did interfere ability immune MIF. Furthermore, were defective could supply “cooperative function” appropriate F 1 mice. results indicate required for soluble antigens and efficient interaction lymphocytes must share some genetic similarities.