Analysis of T cell receptor repertoire based on Vβ chain in patients with breast cancer

摘要: To find out if the T cell repertoire is efficiently and specifically provoked in patients with breast cancer, we have investigated clonotypes of main subsets (based on Vβ-Chain) tumor draining lymph nodes. CD4+ helper, CD8+ cytotoxic (CD4+CD127dimCD25+) regulatory cells, were negatively selected, isolated, from node mononuclear cells 14 untreated cancer. Four non-malignant patients, who underwent surgical operation, also recruited as control group. Based sequences new nomenclature Receptor β Variables (TRBVs) available international ImMunoGeneTics (IMGT) database, 28 TRBV specific forward primers two TRB Constant region (TRBC) reverse developed to amplify all functional alleles. Fluorescent-labeled PCR products then run an ABI PRISM 310 Genetic-Analyzer. The data was analyzed by GeneMapper software version 3.1. Clonotype analysis suggested that activated are present More TRBV usage detected among helper subsets, Gaussian-like pattern majority families; whereas showed oligoclonality almost families one or dominant peaks each family. Similar some these TRBVs observed controls. Having no expression polyclonality controls, oligoclonal TRBV18, however, appears be cancer patients. This phenomenon may reflect existence antigenic stimulation(s) BC preferentially activating those clones express TRBV18.